351 / 2018-07-31 23:14:52

Effects of Three Microbial Inoculation on Mixed Storage Quality and Microbial Community Diversity of Dry Corn Straw and Cabbage Waste

全体主题 > 生物质液体燃料

Corn straw is a common forage and energy crop. However, due to factors such as farming conditions and farming habits in China, most of the corn straw is harvested after wilting and drying, resulting in a large loss of water and sugar and a serious degree of lignification, which limits its resource utilization. China is also a big country in vegetable cultivation and production. The cabbage waste is often abandoned or buried, which causes the pollution of the environment. Therefore, the storage of corn straw and cabbage waste is imminent. The experiment was conducted to study effects of Lactobacillus plantarum, Lactobacillus buchneri and Pichia anomala on mixed storage quality and microbial community diversity of dried corn straw and cabbage waste. The control group ME (mixed storage of dry corn straw and cabbage waste without additive), group LP (mixed storage of DMS and CW with the L.plantarum inoculation), group LB (mixed storage of dry corn straw and cabbage waste with the L.buchneri inoculation) and group PA (mixed storage of dry corn straw and cabbage waste with the P.anomala inoculation ) were set. Four groups were ensiled for 60 d airtightly, the chemical composition and fermentation quality were observed for a 30 d interval, and the microbial community diversity were analyzed by Illumina MiSeq high-throughput sequencing. The results showed that the content of water soluble carbohydrates, dry matter and acetic acid in group LP , LB and PA were increased significantly (P<0.05) compared to group ME. In contrast, the content of acid detergent fiber, holocellulose and pH value in group LP , LB and PA were decreased significantly (P<0.05) compared to group ME during storage for 60 days, and the ratio of ammonia nitrogen to total nitrogen (AN/TN) were decreased significantly (P<0.05) comparing to ME during storage for 30 days. Comparing to group ME, the content of lactic acid increased significantly (P<0.05) in group PA and decreased significantly (P<0.05) in group LP and LB. The ratio of lactic acid to acetic acid (LA/AA) and lactic acid to total organic acid (LA/TOA) were improved by the addition of P.anomala inoculation, which indicating that the intensity of lactic acid fermentation were improved. The dominant bacterium communities in groups LP , LB and PA were Firmicutes and Proteobacteria in phylum level; Lactobacillus was dominant in genus level. The main spoilage bacteria during storage was Enterobacter, and the abundance of Enterobacter in group LP, LB and PA were significantly lower than that in group ME (except group PA of 60 d ). Overall, the findings of this study suggested that adding the microbial inoculants could improve storage quality of mixed silage, and P.anomala was more suitable for practical application.