Torrefaction is a promising pretreatment technology for biomass and its pyrolysis products upgrading. The aim of this study was to investigate the effect of torrefaction on the pyrolysis of seaweed biomass using TG, Py-GC/MS and fixed-bed experiments at a series of typical torrefaction temperatures (225, 250, 275 and 300 °C) and pyrolysis temperatures (450, 500, and 550 °C). The results showed the moisture content and the volatile content were gradually reduced due to due to the precipitation of water and volatile functional groups (OH, NH, CO, C=O, etc.) as the torrefaction temperature increasing, which made the yield of solid product of the torrefied seaweed increase, and the liquid yield showed an promotion first and then decrease. It also indicated that thermal stability of seaweed became stronger after the pretreatment leading to the initial pyrolysis temperature shifting toward the high temperature section. Meanwhile, the maximum pyrolysis weight loss rate and the total weight loss got a reduction. In addition, the optimal condition to produce bio-oil was obtained when the temperature is torrefaction is 250 ° C and the pyrolysis temperature is 500 °C, and the bio-oil production under such conditions was with the highest relative content of aromatic hydrocarbons.