512 / 2018-09-14 16:19:00

Optimization and Kinetics of Mixed Enzymatic Hydrolysis of Acid-explosed Poplar Wood

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Mixed enzymolysis is frequently employed in the bioconversion of lignocellulosic biomass, such as to bioethanol. Wherein, the enzymolysis process optimization and kinetics modeling are of much interest in improving the bioconversion efficiency and control accuracy.  In this paper, the hydrolysis process of acid-explosed poplar wood by mixed cellulase (commercial cellulase appended with β-glucosidase) was optimized and the hydrolysis kinetics model established. The results showed that the optimal enzymolysis parameters were initial pH 5.2, temperature 46 °C and enzymes ratio 1.62, obtaining a highest enzymatic saccharification efficiency of 66.94%, which was 1.2 folds of the unoptimized one. A mixed enzymolysis kinetic model based on Michaelis-Menten equation was established, revealing the interactive mechanism between the substrate concentration and reaction rate. The multiple simultaneous saccharification and co-fermentation (MSSCF) was also conducted for verification of the process optimization efficiency, achieving the highest ethanol concentration of 30.09±0.49 g/l and theoretical ethanol yield of 82.03%, which was 1.64 times higher than the unoptimized one. After optimization, the fermentation time was also reduced by about 24h.