96 / 2019-10-16 11:58:44

Effects of microcystin-LR on the community composition of intestinal microbiota in mice

microcystin-LR,intestinal microbiota,community composition,high-throughput sequencing

Effects of microcystin-LR on the community composition of intestinal microbiota in mice
Wen-Ya Liu a, Fei-Yu Huang a, Hai Feng a, Xiao-Yu Li a, Fei Yang a,*
a Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha, Hunan, China.

ABSTRACT
OBJECTIVE: To provide more data for studying the causes of increased risk of intestinal diseases caused by MC-LR, we observed and clarified the changes of the intestinal flora diversity and community composition of C57 mice.
METHODS: A total of 27 weaned C57 mice were enrolled, in which the intestinal microbiota was still in the stage of establishment. These mice were administrated with MC-LR at concentrations of 0 μg/L, 60 μg/L and 120 μg/L via drinking water for 3 months. The fresh fecal samples of each mouse were collected, and the total genomic DNA were extracted, which were processed according to the company's requirements, and then were sent to the company for high-throughput sequencing using Illumina Miseq high-throughput sequencing method.
RESULTS: Diversity analysis showed that there were no significant differences in the intestinal microbiota diversity of mice fed with different concentrations of MC-LR. Taxonomic analysis showed that the Bacteroides and Firmicutes were the two major dominant phylums of intestinal microflora in all groups. The proportions of micro-bacteria in different goups of mice treated with 60 μg/L and 120 μg/L MC-LR both were 0.3%, which were lower than that of the control group (3.6%). In addition, the relative abundance of the genus Allobaculum in these two treated groups were 1.6% and 1.0% respectively, which were lower than that in the control (4.0%). The relative abundance of the genus Akkermansia were both 0.3%, which were lower than that in the healthy group (3.2%).
Conclusion: Microcystin-LR can cause changes in the gut microbiota composition of mice.
Keywords：microcystin-LR；intestinal microbiota；community composition；high-throughput sequencing

Funding: This work was supported by the National Natural Science Foundation (81773393, 81502787); Central South University Innovation Driven Project (20170027010004)


* Corresponding author at: Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, China.
E-mail address: phfyang@csu.edu.cn