Facing the challenge posed by the prohibition of antibiotics, identifying sustainable and eco-friendly alternatives is crucial. Ampelopsis grossedentata flavonoids, a plant-based extract, possess high antioxidant and anti-inflammatory activities and offer a non-toxic and harmless option for the extended application of additives. This study explored the feasibility of Ampelopsis grossedentata flavonoids as a potential substitute for antibiotics. The research aimed to evaluate the possible impact of supplementing Ampelopsis grossedentata flavonoids on the growth performance, immune competence, and rumen microbial diversity in goats. Consequently, this study provides an important reference for the future development of Ampelopsis grossedentata flavonoids as a new environmentally friendly additive.