15 / 2024-10-09 17:19:02

Design and regulation of NA-targeted AUTAC in G. parasuis Infections and their impact on xenophagy modulation

Glaesserella parasuis (GPS) is a significant bacterial pathogen present in major pig-producing countries worldwide, and the challenges associated with its treatment and antimicrobial resistance have attracted considerable attention from researchers in the industry. This study investigates the changes in virulence and phagocytic resistance of G.parasuis under antibiotic pressure. The findings reveal that resistant strains exhibit increased virulence towards host cells, a change that is associated with lower levels of autophagy in the infected cells. This research suggests that resistant G.parasuis may enhance their survival space by secreting virulence factors that inhibit the phagocytosis of host cells. Furthermore, through transcriptomic analysis, the study identifies NA as a gene that is commonly upregulated in resistant strains and validates its impact on the virulence and phagocytic resistance of G.parasuis through gene knockout experiments. Based on these findings, the study concludes by designing AUTAC molecules specifically targeting NA to investigate their effects on G.parasuis infection, thereby providing new insights and strategies for the development of novel antimicrobial agents.