26 / 2024-10-12 16:03:30

Chloramphenicol Monoclonal Antibody Discovery Based on Precise Selection of Single Transgenic Hybridomas

1.Chloramphenicol is a fungicide and an efficient broad-spectrum antibiotic. It has been added to feed as a veterinary drug. However, long-term consumption of food with excessive chloramphenicol residues may cause intestinal flora imbalance, leading to digestive dysfunction, and even lead to aplastic anemia and thrombocytopenia, liver damage and other health hazards. Therefore, the ' Maximum Residue Limits for Veterinary Drugs in Animal Foods ' stipulates that chloramphenicol is a prohibited drug and cannot be detected in animal food.
2.Nowadays, the enzyme-linked immunosorbent assay for detecting CAP using monoclonal antibodies prepared by LDM is time-consuming and laborious, and the monoclonal antibodies made lack sufficient affinity to meet the needs of trace CAP detection.
3.Here, we describe a single transgenic hybridoma selection method ( STHSM ). The detection method established by the monoclonal antibody prepared by this method has better sensitivity, accuracy and precision.