28 / 2024-10-12 18:47:17

Isolation, identification and application of Staphylococcus pseudointermedius and bacteriophage from companion animals

PURPORT
Staphylococcus pseudointermedius is a common opportunistic pathogen in companion animals. The overuse of antibiotics leads to the emergence of methicillin-resistant staphylococcus pseudointermedius. Companion animals share a large amount of living space with humans, so drug-resistant strains of Staphylococcus pseudointermedius exist for transmission between owners and companion animals. Bacteriophages, as viruses of bacteria, can specifically kill host bacteria and have high sterilization efficiency, which is a very promising biological product in the development of antibacterial drugs instead of anti biotics. The purpose of this study is to isolate and culture pseudomorph intermedius bacteriophages and develop therapeutic drugs instead of antibiotics on the basis of them, so as to provide a new solution for preventing public health problems caused by companion animal breeding.
Purpose of resrech
To provide a theoretical basis for the use of phages in the treatment of drug-resistant bacterial infections in companion animals under the background of reducing and replacing resistance.
Research methods
Dermatitis samples were collected from companion animals for isolation and identification of pathogenic bacteria, and bacteriophages were isolated using the isolated pathogenic bacteria as host. The bacteriophage cocktail was prepared by mixing the isolated bacteriophage, and the hydrogel preparation was made after verifying its bacteriostatic effect. Then the therapeutic effect of the bacteriophage cocktail hydrogel was evaluated and clinical trials were conducted.
Excped result
Multiple strains of pseudostaphylococcus intermedius could be isolated and used to produce a phage cocktail. The host spectrum of the phage cocktail could cover common clinical strains of pseudostaphylococcus intermedius and Staphylococcus aureus. Then it can be successfully introduced into the hydrogel, on the basis of the original anti-inflammation and pro-healing effect of the hydrogel to form a new high-efficiency antibacterial, anti-inflammatory and pro-healing product. This experiment will provide a pioneering idea for the development of new wound dressings by introducing phages into other materials, and provide a new solution for the treatment of drug-resistant bacterial infections using non-antibiotics in the environment of "reducing resistance and replacing resistance".
Slogan
Love around the earth, to "phage" to share the future without resistance!