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Development of Recombinant Chimeric Porcine Epidemic Diarrhea Virus via Reverse Genetics Techniques and Its Clinical Evaluation

Porcine epidemic diarrhea virus (PEDV) is one of the most serious pathogens affecting the health of piglets (3-10-day-old). Clinically, PEDV mutates rapidly and is difficult to isolate, resulting in a relatively low immune protection rate of existing vaccines. In this study, we constructed a recombinant virus backbone with reverse genetics technology . Taking the cell-adapted strain of PEDV as the backbone, through molecular cloning techniques such as homologous recombination and mutagenic PCR, we constructed a recombinant chimeric virus of the PEDV strain that is difficult to isolate clinically. We evaluated the safety of the recombinant virus in newborn piglets, which lay the foundation for the rapid development of an effective vaccine against clinically difficult-to-isolate PEDV strains