54 / 2024-10-23 15:36:22

The Fe3O4@GO/Aptamer magnetic carbon nanoscale multifunctional probe-based ATP bioluminescence detection technology for the contamination and drug resistance of important foodborne pathogens

Persistent contamination of foodborne pathogens with the emergence of antimicrobial resistance is a serious threat to food safety and public health security. It is extremely important to monitor the contamination and drugs resistance of foodborne pathogens. The existing detection technologies for various foodborne pathogens are not enough sensitive and specific, and there are few studies on monitoring contamination and drug resistance at the same time. Therefore, this study will construct a Fe3O4@GO/Aptamer magnetic carbon nanoscale multifunctional probe-based ATP bioluminescence detection technology for contamination and antimicrobial resistance of seven kinds of foodborne pathogens. The main research contents includes: ①The modification of aptamers active sites for various foodborne pathogens will be performed to increase the affinity of aptamers to target pathogens. ②The Fe3O4@GO/Aptamer magnetic carbon nanoscale aptamer probe will be prepared to specifically identify and enrich seven kinds of foodborne pathogens; ③The working conditions of laser will be optimized to increase the photothermal lysis bacteria efficiency and release ATP in bacteria effectively. ④The optimization of microbial culture system’s parameters and ATP bioluminescence system’s parameters will be performed to develop ATP bioluminescence detection technology. Accordingly, the research results of this study will provide a promising monitoring scheme for the specific and sensitive detection of food-borne pathogens contamination and drug resistance, which will have important significance for the ensurement of food safety and public health security.