67 / 2024-10-24 23:03:07

Development of a Multi-Indicator Rapid Detection System Based on Microfluidics Technology and Rapid Pathogen Identification Using RPA Single Test and RPA-CRISPR Integration

With the expansion of aquaculture, the frequency of diseases caused by aquatic pathogens has increased, leading to significant losses in the aquaculture industry. Meanwhile, waterborne pathogens and zoonotic pathogens, through environmental pollution and contamination of coastal waters, pose a serious threat to public health.
Isothermal amplification, known for its speed and simplicity, has been widely used in detecting pathogenic microorganisms. And integration with CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) improves precision in identifying highly homologous species while maintaining simplicity and convenience. Furthermore, integration with microfluidic chips offers enhanced efficiency for handling large amounts of samples or performing parallel analysis of multiple targets.
Ensuring the effectively monitor aquaculture diseases and zoonoses, detecting specific microorganisms in water bodies and controlling their spread are crucial for ensuring the health of aquatic animals, humans, and maintaining the health of the water environment. These measures are significant for promoting the implementation of the "One Health" concept.