75 / 2024-10-25 10:19:40

Study on the mechanism of escape inhibition and neutralization effect of inhibitors at key sites of NADC30-LIKE PRRSV GP5 protein

PRRSV causes reproductive failure, including stillbirth and autolyzed and mummified fetus in sows, as well as respiratory disease leading to fever, severe dyspnea, anorexia, and lethargy in growing pigs.Among currently commercially available attenuated live vaccines, type Ⅰ PRRSV infection has a better protective effect, while type Ⅰ and Ⅱ vaccines can only provide less cross-protection.
It is reported that antibodies against GP5 can effectively neutralize PRRSV. We compared the GP5 amino acid sequences of two strains of PRRSV, and found that the two GP5s have multiple amino acid differences near the neutralization site. In this study, PRRSV-ZJ/JX/2015 virus was used as the backbone to construct a recombinant chimeric virus through homologous recombination and mutation PCR, and its safety, stability and immune protection effect were evaluated in newborn piglets, providing a basis for the development of antibodies against GP5.