Antimicrobial resistance (AMR) in livestock-derived and poultry-derived Salmonella is a considerable public health risk in China. However, limited information is available on the prevalence of AMR and virulence genes in Salmonella among food-producing animals in Central China.The objective of this study was to assess the prevalence of AMR, virulence genes, mobile genetic elements, and multilocus sequence typing (MLST) distribution in Salmonella isolated from swine, chicken, and cattle in Central China. Furthermore, the molecular mechanism of AMR and pathogenicity of the bacteria were explored. PCR was used to assess 187 epidemiologically independent AMR isolates from three provinces for AMR and virulence genes.