Following developments in device technologies, medical data collected by a variety of modalities provide a wealth of information for diagnosis and other purposes. Clearly, that information is only of use if it has been extracted, processed and analysed appropriately. The International Workshop on Medical Image and Signal Analysis (MISA 2013) aims to provide a forum for current state-of-the-art research that adresses these issues and focusses on medical image and data processing and analysis and related applications.